Article
The two-step strategy for enhancing the specific activity and thermostability of alginate lyase AlyG2 with mechanism for improved thermostability.
International journal of biological macromolecules - 1 Jul 2024
Huang Jin-Ping, Yun Shuai-Ting, Zhao Jin-Xin, Wang Xue-Ting, Wang Xiao-Chen, Guo Xiang-Yi, San Dong-Mei, Zhou Yan-Xia
Abstract excerpt
To overcome the trade-off challenge encountered in the engineering of alginate lyase AlyG2 from Seonamhaeicola algicola Gy8T and to expand its potential industrial applications, we devised a two-step strategy encompassing activity enhancement followed by thermal stability engineering. To enhance the specific activity of efficient AlyG2, we strategically substituted residues with bulky steric hindrance proximal to...
Topics
- Polysaccharide-Lyases
- Hot Temperature
- Enzyme Stability
- Biocatalysis
- Hydrogen Bonding
- Substrate Specificity
- Models, Molecular
- Protein Structure, Tertiary
- Mutation
- Catalytic Domain