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Article

The two-step strategy for enhancing the specific activity and thermostability of alginate lyase AlyG2 with mechanism for improved thermostability.

International journal of biological macromolecules - 1 Jul 2024

Huang Jin-Ping, Yun Shuai-Ting, Zhao Jin-Xin, Wang Xue-Ting, Wang Xiao-Chen, Guo Xiang-Yi, San Dong-Mei, Zhou Yan-Xia

Abstract excerpt

To overcome the trade-off challenge encountered in the engineering of alginate lyase AlyG2 from Seonamhaeicola algicola Gy8T and to expand its potential industrial applications, we devised a two-step strategy encompassing activity enhancement followed by thermal stability engineering. To enhance the specific activity of efficient AlyG2, we strategically substituted residues with bulky steric hindrance proximal to...

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PMID: 38823749
DOI: 10.1016/j.ijbiomac.2024.132685

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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