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Enhancement of the Thermostability of Microbacterium Esterase by Combinatorial Rational Design.

Molecules (Basel, Switzerland) - 11 Dec 2024

Peng Wenyu, Wu Xiaomei, Ma Baodi, Xu Yi

Abstract excerpt

The esterase EstSIT01 from Microbacterium can catalyze the asymmetric hydrolysis of meso-dimethyl ester to produce the crucial chiral intermediate (4S, 5R)-hemimethyl ester for d-biotin synthesis. Despite its high yields and stereoselectivity, the low thermostability of EstSIT01 limits its practical application. Herein, two kinds of rational strategies were combined to enhance the thermostability of EstSIT01....

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PMID: 39769928
DOI: 10.3390/molecules29245839

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