Article
Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing.
Nature communications - 3 Mar 2026
Becerra Basheer, Wittibschlager Sandra, Patel Zain M, Kutschat Ana P, Delano Justin, Che Eric, Tauber Anzhelika, Wu Ting, Starrs Marlena, Horstmann Christina S, Müller Sophie, Whittaker Madelynn N, Sylvander Elise, Lehner Manfred, Love Michael I, Kleinstiver Benjamin P, Jankowiak Martin, Bauer Daniel E, Seruggia Davide, Pinello Luca
Abstract excerpt
CRISPR tiling screens have enabled the characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing and enrichment analysis. This study introduces an end-to-end experimental assay and computational pipeline, which leverages targeted sequencing of CRISPR-introduced alleles at the endogenous target locus following dense base-editing...
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