Article
Base-editing mutagenesis maps alleles to tune human T cell functions.
Nature - 1 Jan 2024
Schmidt Ralf, Ward Carl C, Dajani Rama, Armour-Garb Zev, Ota Mineto, Allain Vincent, Hernandez Rosmely, Layeghi Madeline, Xing Galen, Goudy Laine, Dorovskyi Dmytro, Wang Charlotte, Chen Yan Yi, Ye Chun Jimmie, Shy Brian R, Gilbert Luke A, Eyquem Justin, Pritchard Jonathan K, Dodgson Stacie E, Marson Alexander
Abstract excerpt
CRISPR-enabled screening is a powerful tool for the discovery of genes that control T cell function and has nominated candidate targets for immunotherapies1-6. However, new approaches are required to probe specific nucleotide sequences within key genes. Systematic mutagenesis in primary human T cells could reveal alleles that tune specific phenotypes. DNA base editors are powerful tools for introducing targeted...
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