Article
A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries.
Biomolecules - 25 Oct 2025
Lu Yuxin, Meng Shuting, Guan Xinyi, He Pengying, Zhao Dongxin
Abstract excerpt
As synthetic biology advances toward precise design, the construction of high-quality mutant libraries has become essential for large-scale functional screening. Traditional approaches, such as random and saturation mutagenesis, often suffer from low accuracy, high bias, and limited coverage. An ideal method should offer controlled mutagenesis, comprehensive coverage, high throughput, operational simplicity, and...
Topics
- Gene Library
- Mutagenesis
- DNA-Directed DNA Polymerase
- Oligonucleotides
- High-Throughput Screening Assays
- Mutation