PyVADesign: a python-based cloning tool for one-step generation of large mutant libraries.

Abstract excerpt

MOTIVATION: The generation and analysis of diverse mutants of a protein is a powerful tool for understanding protein function. However, generating such mutants can be time-consuming, while the commercial option of buying a series of mutant plasmids can be expensive. In contrast, the insertion of a synthesized double-stranded DNA (dsDNA) fragment into a plasmid is a fast and low-cost method to generate a large...

Topics

• Software
• Cloning, Molecular
• Gene Library
• Plasmids
• Mutation
• DNA