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Article

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids.

New biotechnology - 25 Dec 2022

Jia Huiyong, Couto-Rodriguez Ricardo, Johnson Sharon, Medina Stephanie, Novillo Brianna, Huynh Peter, Kim Matt, Cooper Cade, Michalik Meagan, Siew Benjamin, Turesson Elise, Maupin-Furlow Julie A

Abstract excerpt

Advances are needed in the site-directed mutagenesis of large plasmids for protein structure-function studies, as current methods are often inefficient, complicated and time-consuming. Here two new methods are reported that overcome these difficulties, namely the single primer extension reaction (SSPER) strategy that reaches 100% efficiency and the reduce recycle PCR (rrPCR) method that is advantageous in...

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Identifiers

PMID: 36007808
DOI: 10.1016/j.nbt.2022.08.004
PMCID: PMC9742187

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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