CodonMutator: a python-based automated oligonucleotide design framework for deep mutational scanning library construction.

Abstract excerpt

Deep mutational scanning (DMS) enables systematic evaluation of protein sequence-function relationships, but its utility is often limited by the complexity of library construction. Existing mutagenesis strategies are either biased, labor-intensive, or prone to design errors, restricting their scalability for comprehensive variant generation. Here, we present a systematic cloning framework coupled with an...

Topics

• Gene Library
• Oligonucleotides
• Mutation
• Cloning, Molecular
• Codon
• High-Throughput Nucleotide Sequencing
• Mutagenesis
• Software