CodonMutator: a python-based automated oligonucleotide design framework for deep mutational scanning library construction.

Abstract excerpt

Deep mutational scanning (DMS) enables systematic evaluation of protein sequence-function relationships, but its utility is often limited by the complexity of library construction. Existing mutagenesis strategies are either biased, labor-intensive, or prone to design errors, restricting their scalability for comprehensive variant generation. Here, we present a systematic cloning framework coupled with an...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cloning, Molecular
• Share to Codon
• Share to Gene Library