PCR-based strategy for construction of multi-site-saturation mutagenic expression library.

doi:10.1016/j.mimet.2007.09.001

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Article

PCR-based strategy for construction of multi-site-saturation mutagenic expression library.

Journal of microbiological methods - 1 Dec 2007

Wang Jinxia, Zhang Sufang, Tan Haidong, Zhao Zongbao Kent

Abstract excerpt

There is an increasing demand for efficient and effective methods to engineer protein variants for industrial applications, structural biology and drug development. We describe a PCR-based strategy that produces multi-site-saturation mutagenic expression library using a circular plasmid carrying the wild-type gene. This restriction digestion- and ligation-independent method involves three steps: 1) synthesis of...

Topics

Cloning, Molecular
DNA Primers
Escherichia coli
Gene Library
Genetic Engineering
Molecular Biology
Mutagenesis, Site-Directed
Mutation
Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 17936385
DOI: 10.1016/j.mimet.2007.09.001

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