Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Plasmid-based one-pot saturation mutagenesis.

Nature methods - 1 Nov 2016

Wrenbeck Emily E, Klesmith Justin R, Stapleton James A, Adeniran Adebola, Tyo Keith E J, Whitehead Timothy A

Abstract excerpt

Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amidohydrolases
Share to beta-Lactamases
Share to DNA Restriction Enzymes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Library

Share to Genes, Bacterial

Share to Mutagenesis, Site-Directed

Share to Plasmids

Share to Pseudomonas aeruginosa

Source

Open in PubMed Open full text

Identifiers

PMID: 27723752
DOI: 10.1038/nmeth.4029
PMCID: PMC5666567

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Plasmid-based one-pot saturation mutagenesis.PMID 27723752Read ↗

CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes.Semantic proximity Read ↗

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Semantic proximity Read ↗

Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method.Semantic proximity Read ↗

MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.Semantic proximity Read ↗

SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries.Semantic proximity Read ↗

TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.Semantic proximity Read ↗

Single-base sequencing for rapid screening of plasmids for inserts with known mutations and correct orientation.Semantic proximity Read ↗

PCR-based strategy for construction of multi-site-saturation mutagenic expression library.Semantic proximity Read ↗

A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries.Semantic proximity Read ↗

Recent advances in targeted mutagenesis to expedite the evolution of biological systems.Semantic proximity Read ↗

Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.Semantic proximity Read ↗

Simple-MSSM: a simple and efficient method for simultaneous multi-site saturation mutagenesis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.