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Article

A cost-effective and scalable barcoded library construction method for deep mutational scanning studies.

PLoS biology - 1 Feb 2026

Jann Jessica, Gagnon-Arsenault Isabelle, Pageau Alicia, Dubé Alexandre K, Fijarczyk Anna, Durand Romain, Landry Christian R

Abstract excerpt

Recent developments in DNA synthesis and sequencing allow the construction of comprehensive gene variant libraries and their functional analysis. Achieving high-replication and thorough mutation characterization remains technically and financially challenging for long genes. Here, we developed an efficient, affordable, and scalable library construction approach that relies on low-cost DNA synthesis and standard...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41671286
DOI: 10.1371/journal.pbio.3003645
PMCID: PMC12923136

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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