Article
A versatile, simple, and rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications.
Methods (San Diego, Calif.) - 1 Jul 2026
Sethumadhavan Kavitha Modenkattil, Kandpal Gayatri, Sajimon Jyothilakshmi, Das V R Akshay, Basu Nirmegh, Simla Athulya Prakash, Raj V Stalin
Abstract excerpt
Gene cloning, site-directed mutagenesis (SDM), and short gene synthesis are essential tools in molecular biology, yet existing approaches remain constrained by efficiency, flexibility, and cost. Here, we developed a versatile, simple, and rapid (VSR) 3-in-1 method that integrates seamless cloning, mutagenesis, and short gene assembly in a single platform. This approach works on the principle of overlap extension...
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