MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.

Abstract excerpt

The editing of plasmids and construction of plasmid libraries is paramount to the engineering of desired functionalities in synthetic biology. Typically, plasmids with targeted mutations are produced through time- and resource-consuming DNA amplification and/or cloning steps. In this study, we establish MOSAIC, a highly efficient protocol for the editing of plasmids and generation of combinatorial plasmid...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to DNA, Single-Stranded
• Share to Escherichia coli
• Share to Gene Editing