Article
Engineering a High-Fidelity MAD7 Variant with Enhanced Specificity for Precision Genome Editing via CcdB-Based Bacterial Screening.
Biomolecules - 4 Oct 2025
Zhang Haonan, Yang Ying, Yang Tianxiang, Cao Peiyao, Yu Cheng, Liang Liya, Liu Rongming, Chen Zhiying
Abstract excerpt
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) nucleases enable precise genome editing, but off-target cleavage remains a critical challenge. Here, we report the development of MAD7_HF, a high-fidelity variant of the MAD7 nuclease engineered through a bacterial screening system leveraging the DNA gyrase-targeting toxic gene ccdB. This system couples survival to...
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