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Hairpin Structure Facilitates Multiplex High-Fidelity DNA Amplification in Real-Time Polymerase Chain Reaction.

Analytical chemistry - 12 Jul 2022

Zhang Kerou, Pinto Alessandro, Cheng Lauren Yuxuan, Song Ping, Dai Peng, Wang Michael, Rodriguez Luis, Weller Cailin, Zhang David Yu

Abstract excerpt

Clinically and biologically, it is essential to detect rare DNA-sequence variants for early cancer diagnosis or drug-resistance mutation identification. Some of the common quantitative polymerase chain reaction (qPCR)-based variant detection methods are restricted in the limit of detection (LoD) because the DNA polymerases used for these methods have a high polymerase misincorporation rate; thus, the detection...

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PMID: 35749270
DOI: 10.1021/acs.analchem.2c00575

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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