Article

Single Tube qPCR detection and quantitation of hotspot mutations down to 0.01% VAF

2021-08-13

Abstract excerpt

<h4>ABSTRACT</h4> Clinically and biologically, rare DNA sequence variants are significant and informative. However, existing detection technologies are either complex in workflow, or restricted in the limit of detection (LoD), or do not allow for multiplexing. Blocker displacement amplification (BDA) method can stably and effectively detect and enrich multiple rare variants with LoD around 0.1% variant allele fra...

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Literature Corpus work: 13ab1c1f-9af2-567e-b025-337d4e253e50
DOI: 10.1101/2021.08.12.456178

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