Article
Allele-specific, non-extendable primer blocker PCR (AS-NEPB-PCR) for DNA mutation detection in cancer.
The Journal of molecular diagnostics : JMD - 1 Jan 2013
Wang Haiying, Jiang John, Mostert Bianca, Sieuwerts Anieta, Martens John W M, Sleijfer Stefan, Foekens John A, Wang Yixin
Abstract excerpt
Allele-specific amplification, combined with TaqMan probe real-time polymerase chain reaction (real-time AS-PCR), has been widely used for detecting genetic variants, single nucleotide polymorphisms, and genetic mutations. In addition, several probe-blocking methods have been introduced in real-time AS-PCR to block amplification of wild-type templates and to increase detection sensitivity and specificity....
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