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Extended Enrichment for Ultrasensitive Detection of Low-Frequency Mutations by Long Blocker Displacement Amplification.

Angewandte Chemie (International ed. in English) - 6 May 2024

Si Yunpei, Wang Xiawen, Su Xinglei, Weng Zhi, Hu Qiongzheng, Li Qian, Fan Chunhai, Zhang David Yu, Wang Yunshan, Luo Shihua, Song Ping

Abstract excerpt

Detecting low-frequency DNA mutations hotspots cluster is critical for cancer diagnosis but remains challenging. Quantitative PCR (qPCR) is constrained by sensitivity, and allele-specific PCR is restricted by throughput. Here we develop a long blocker displacement amplification (LBDA) coupled with qPCR for ultrasensitive and multiplexed variants detection. By designing long blocker oligos to perfectly match...

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PMID: 38416545
DOI: 10.1002/anie.202400551

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