Article

Simple and Multiplexed Enrichment of Rare DNA Variants via Sequence-Selective and Temperature-Robust Amplification

2017-05-10

Abstract excerpt

Rare DNA sequence variants hold important clinical and biological information, but are chal-lenging for existing methods (e.g. PCR, NGS) to profile in an inexpensive, multiplexed, simple-to-implement, and sequence-general way. Here, we present Blocker Displacement Amplification (BDA), a temperature-robust PCR method that selectively amplifies all sequence variants within a roughly 20 nt window by 1000-fold over wi...

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Literature Corpus work: b03f3a17-5379-5c0e-9923-bb2f1efd1fb6
DOI: 10.1101/136465

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