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Article

Highly Sensitive Blocker Displacement Amplification for Detection of Low-Level JAK2V617F Variant.

The journal of applied laboratory medicine - 4 May 2023

Wang Zhen, Weller Cailin, Pinto Alessandro, Zhang David Yu, Mularo Frank, Tu Zheng Jin, Cheng Yu-Wei

Abstract excerpt

BACKGROUND: Key criteria in the diagnostic workup and risk stratification for myeloproliferative neoplasms (MPN) include molecular testing for JAK2V617F and other mutant alleles. Multiple methods for quantitatively detecting nucleotide sequence changes exist, but the lower limit of detection can limit identification of the low-level allele fraction of a variant. We evaluated a recently developed blocker...

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PMID: 36759689
DOI: 10.1093/jalm/jfac130

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