Article

Hairpin structure facilitates multiplex high-fidelity DNA amplification in real-time PCR

2021-11-14

Abstract excerpt

Clinically and biologically, it is essential to detect rare DNA-sequence variants for early cancer diagnosis or drug-resistance mutations identification. Some of the common quantitative PCR (qPCR)-based variant detection methods are restricted in the limit of detection (LoD) because the DNA polymerases used have a high polymerase misincorporation rate, thus the detection sensitivity is sometimes unsatisfactory. Wi...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d397e402-d306-5867-908e-31117a53cd7e
DOI: 10.1101/2021.11.10.21266179

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Hairpin structure facilitates multiplex high-fidelity DNA amplification in real-time PCRDOI 10.1101/2021.11.10.21266179Read

Hairpin Structure Facilitates Multiplex High-Fidelity DNA Amplification in Real-Time Polymerase Chain Reaction.Semantic proximity Read

Highly Sensitive Enrichment of Low-Frequency Variants by Hairpin Competition Amplification.Semantic proximity Read

Ribonucleotide-Modified Primer Overlapping Blocker Elongation (PROBE) Assay for Highly Specific Mutation Detection.Semantic proximity Read

Extended Enrichment for Ultrasensitive Detection of Low-Frequency Mutations by Long Blocker Displacement Amplification.Semantic proximity Read

Structure-Switchable Hairpin-Powered Exponential Replications for Sensing Attomolar microRNA-Related Single Nucleotide Polymorphisms in Human Cancer Tissues with Zero Background.Semantic proximity Read

Modified Unit-Mediated Strand Displacement Reactions for Direct Detection of Single Nucleotide Variants in Active Double-Stranded DNA.Semantic proximity Read

Cost-Efficient Sequence-Based Nonextensible Oligonucleotide in Real-Time PCR and High-Throughput Sequencing.Semantic proximity Read

Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants.Semantic proximity Read

Hairpin-Empowered Invasive Reaction Combined with Catalytic Hairpin Assembly Cascade Amplification for the Specific Detection of Single-Nucleotide Polymorphisms.Semantic proximity Read

Simple and Multiplexed Enrichment of Rare DNA Variants via Sequence-Selective and Temperature-Robust AmplificationSemantic proximity Read

Single-Tube qPCR Detection and Quantitation of Hotspot Mutations Down to 0.01% Variant Allele Fraction.Semantic proximity Read

Allele-specific, non-extendable primer blocker PCR (AS-NEPB-PCR) for DNA mutation detection in cancer.Semantic proximity Read