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Identification of DNA variants at ultra-low variant allele frequencies via Taq polymerase cleavage of wild-specific blockers.

Analytical and bioanalytical chemistry - 1 Nov 2023

Liu Zhaocheng, Li Xiushuai, Zhang Rui, Ji Li, Gong Lingli, Ji Yong, Zhou Fengsheng, Yin Ying, Li Koukou, Sun Ping, Pu Zhening, Wang Qing, Zou Jian

Abstract excerpt

Detecting mutations related to tumors holds immense clinical significance for cancer diagnosis and treatment. However, the presence of highly redundant wild DNA poses a challenge for the advancement of low-copy mutant ctDNA genotyping in cancer cases. To address this, a Taqman qPCR strategy to identify rare mutations at low variant allele fractions (VAFs) has been developed. This strategy combines mutant-specific...

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PMID: 37702773
DOI: 10.1007/s00216-023-04931-0

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UBC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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