Detection system- and strain-dependent diversity of de novo [PSI+] prion generation and phenotypes in Saccharomyces cerevisiae.

Abstract excerpt

Yeast prion [PSI+], an amyloid form of the translation termination factor Sup35p/eRF3, causes translational stop codon readthrough by sequestering functional Sup35p. This unique phenotype may be analyzed via [PSI+]-suppressible nonsense alleles, and has greatly contributed to the advancement in yeast prion research. For comparing canonical reporters, like chromosomal ade1-14 or ade2-1, and plasmid-borne ura3-14,...

Topics

• Saccharomyces cerevisiae
• Saccharomyces cerevisiae Proteins
• Peptide Termination Factors
• Prions
• Phenotype
• Codon, Nonsense