Residues neighboring an SH3-binding motif participate in the interaction in vivo.

Abstract excerpt

In signaling networks, protein-protein interactions are often mediated by modular domains that bind short linear motifs. The motifs' sequences affect many factors, among them affinity and specificity, or the ability to bind strongly and to the appropriate partners. Using Deep Mutational Scanning to create a mutant library, and protein complementation assays to measure protein-protein interactions, we determined...

Topics

• Saccharomyces cerevisiae Proteins
• src Homology Domains
• Saccharomyces cerevisiae
• Protein Binding
• Amino Acid Motifs
• Binding Sites
• Mutation
• Models, Molecular