Quantification of the transferability of a designed protein specificity switch reveals extensive epistasis in molecular recognition.

Abstract excerpt

Reengineering protein-protein recognition is an important route to dissecting and controlling complex interaction networks. Experimental approaches have used the strategy of "second-site suppressors," where a functional interaction is inferred between two proteins if a mutation in one protein can be compensated by a mutation in the second. Mimicking this strategy, computational design has been applied...

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