Article
Combinatorial deep mutational scanning uncovers protein superbinders and molecular determinants of epistasis.
Molecular cell - 17 Sept 2026
Jiang Mingxuan, Sun Mohan, Cheng Nuo, Örd Mihkel, Augustin Teresa L, Li Allyson, Shah Neel H, Rinehart Jesse, Mott Helen R, Creixell Pau
Abstract excerpt
Traditional deep mutational scanning (DMS) encodes every single amino-acid substitution from a wild-type sequence. We hypothesize that combinatorial DMS (CDMS) libraries, incorporating all mutations in all combinations, can enable the discovery of high-affinity protein (super)binders by capturing epistatic, non-linear amino-acid interactions. Here, we introduce origin-independent and context-exhaustive...
Read the complete abstract on PubMedTopics
- Epistasis, Genetic
- Protein Binding
- NIMA-Interacting Peptidylprolyl Isomerase
- Mutation
- Peptide Library
- Models, Molecular