Article
High-coverage allele-resolved single-cell DNA methylation profiling reveals cell lineage, X-inactivation state, and replication dynamics.
Nature communications - 8 Jul 2025
Spix Nathan J, Habib Walid Abi, Zhang Zhouwei, Eugster Emily, Milliron Hsiao-Yun, Sokol David, Lee KwangHo, Nolte Paula A, Endicott Jamie L, Krzyzanowski Kelly F, Hinoue Toshinori, Morrison Jacob, Johnson Benjamin K, Zhou Wanding, Shen Hui, Laird Peter W
Abstract excerpt
DNA methylation patterns at crucial short sequence features, such as enhancers and promoters, may convey key information about cell lineage and state. The need for high-resolution single-cell DNA methylation profiling has therefore become increasingly apparent. Existing single-cell whole-genome bisulfite sequencing (scWGBS) studies have both methodological and analytical shortcomings. Inefficient library...
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