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Article

Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells.

Nucleic acids research - 2 Jun 2017

Han Lin, Wu Hua-Jun, Zhu Haiying, Kim Kun-Yong, Marjani Sadie L, Riester Markus, Euskirchen Ghia, Zi Xiaoyuan, Yang Jennifer, Han Jasper, Snyder Michael, Park In-Hyun, Irizarry Rafael, Weissman Sherman M, Michor Franziska, Fan Rong, Pan Xinghua

Abstract excerpt

Conventional DNA bisulfite sequencing has been extended to single cell level, but the coverage consistency is insufficient for parallel comparison. Here we report a novel method for genome-wide CpG island (CGI) methylation sequencing for single cells (scCGI-seq), combining methylation-sensitive restriction enzyme digestion and multiple displacement amplification for selective detection of methylated CGIs. We...

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Identifiers

PMID: 28126923
DOI: 10.1093/nar/gkx026
PMCID: PMC5605247

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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