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Article

Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.

Communications biology - 1 Feb 2021

Niemöller Christoph, Wehrle Julius, Riba Julian, Claus Rainer, Renz Nathalie, Rhein Janika, Bleul Sabine, Stosch Juliane M, Duyster Justus, Plass Christoph, Lutsik Pavlo, Lipka Daniel B, Lübbert Michael, Becker Heiko

Abstract excerpt

Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33526904
DOI: 10.1038/s42003-021-01661-w
PMCID: PMC7851132

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