Precise measurement of molecular phenotypes with barcode-based CRISPRi systems.

Abstract excerpt

Genome-wide CRISPR-Cas9 screens have untangled regulatory networks driving diverse biological processes. Their success relies on interrogating specific molecular phenotypes and distinguishing key regulators from background effects. Here, we realize these goals by optimizing CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) to dramatically improve the sensitivity and scope of genome-wide...

Topics

• CRISPR-Cas Systems
• Phenotype
• Humans
• Promoter Regions, Genetic