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Article

Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation.

eLife - 16 Nov 2020

Brion Christian, Lutz Sheila M, Albert Frank Wolfgang

Abstract excerpt

Trans-acting DNA variants may specifically affect mRNA or protein levels of genes located throughout the genome. However, prior work compared trans-acting loci mapped in separate studies, many of which had limited statistical power. Here, we developed a CRISPR-based system for simultaneous quantification of mRNA and protein of a given gene via dual fluorescent reporters in single, live cells of the yeast...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 33191917
DOI: 10.7554/eLife.60645
PMCID: PMC7707838

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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