Article
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes.
Science (New York, N.Y.) - 11 Dec 2020
Muller Ryan, Meacham Zuriah A, Ferguson Lucas, Ingolia Nicholas T
Abstract excerpt
To realize the promise of CRISPR-Cas9-based genetics, approaches are needed to quantify a specific, molecular phenotype across genome-wide libraries of genetic perturbations. We addressed this challenge by profiling transcriptional, translational, and posttranslational reporters using CRISPR interference (CRISPRi) with barcoded expression reporter sequencing (CiBER-seq). Our barcoding approach allowed us to...
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