Article

A Two-tiered Functional Screen Identifies Herpesviral Transcriptional Modifiers and their Essential Domains

2021-09-22

Abstract excerpt

While traditional methods for studying large DNA viruses allow the creation of individual mutants, CRISPR/Cas9 can be used to rapidly create thousands of mutant dsDNA viruses in parallel. Here, we used this approach to study the human oncogenic Kaposi’s sarcoma-associated herpesvirus (KSHV). We designed a sgRNA library containing all possible ~22,000 guides targeting the genome of KSHV – one cut site approximately...

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Literature Corpus work: abce3411-c399-5509-b338-9268127afa03
DOI: 10.1101/2021.09.20.460982

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