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A Massively Parallel In Vivo Assay of TdT Mutants Yields Variants with Altered Nucleotide Insertion Biases.

ACS synthetic biology - 18 Oct 2024

Carlson Courtney K, Loveless Theresa B, Milisavljevic Marija, Kelly Patrick I, Mills Jeremy H, Tyo Keith E J, Liu Chang C

Abstract excerpt

Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase capable of template-independent extension of DNA. TdT's de novo DNA synthesis ability has found utility in DNA recording, DNA data storage, oligonucleotide synthesis, and nucleic acid labeling, but TdT's intrinsic nucleotide biases limit its versatility in such applications. Here, we describe a multiplexed assay for profiling and engineering...

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Identifiers

PMID: 39302688
DOI: 10.1021/acssynbio.4c00414

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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