Article
Enhanced Taq Variant Enables Efficient Genome Editing Testing and Mutation Detection.
The CRISPR journal - 1 Feb 2022
Du Ping, Li Bo, Liu Xiaodan, Yang Lele, Ren Naixia, Li Yingying, Huang Qilai
Abstract excerpt
Detection of genome editing with quantitative polymerase chain reaction (PCR) primarily relies on and is limited by its ability to discriminate genome modification from the wild-type sequence. An enhanced DNA polymerase variant with superior specificity is needed for this application. Here, we perform semi-rational molecular evolution on full-length Taq polymerase to screen high-specific variants that meet the...
Topics
- Alleles
- CRISPR-Cas Systems
- Gene Editing
- Mutation
- Taq Polymerase