Article

Prime editor-based high-throughput screening reveals functional synonymous mutations in the human genome

2024-06-17

Abstract excerpt

Synonymous mutations are generally considered neutral, while their roles in the human genome remain largely unexplored. Herein, we employed the PEmax system to create a library of 297,900 epegRNAs and performed extensive screening to identify synonymous mutations that impact cell fitness. While most synonymous mutations are neutral, we found that some can elicit phenotypic effects. By developing a specialized mach...

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Literature Corpus work: b52a3909-4b26-5a97-b5ee-de0520daec9c
DOI: 10.1101/2024.06.16.599253

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