Article
Systematic Exploration of Optimized Base Editing gRNA Design and Pleiotropic Effects with BExplorer.
Genomics, proteomics & bioinformatics - 1 Dec 2023
Zhang Gongchen, Zhu Chenyu, Chen Xiaohan, Yan Jifang, Xue Dongyu, Wei Zixuan, Chuai Guohui, Liu Qi
Abstract excerpt
Base editing technology is being increasingly applied in genome engineering, but the current strategy for designing guide RNAs (gRNAs) relies substantially on empirical experience rather than a dependable and efficient in silico design. Furthermore, the pleiotropic effect of base editing on disease treatment remains unexplored, which prevents its further clinical usage. Here, we presented BExplorer, an integrated...
Topics
- Gene Editing
- Humans
- Polymorphism, Single Nucleotide
- RNA, Guide, CRISPR-Cas Systems
- Genome, Human
- Genetic Pleiotropy
- CRISPR-Cas Systems
- Software
- Computational Biology