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Article

High-throughput evaluation of genetic variants with prime editing sensor libraries.

Nature biotechnology - 1 Oct 2025

Gould Samuel I, Wuest Alexandra N, Dong Kexin, Johnson Grace A, Hsu Alvin, Narendra Varun K, Atwa Ondine, Levine Stuart S, Liu David R, Sánchez Rivera Francisco J

Abstract excerpt

Tumor genomes often harbor a complex spectrum of single nucleotide alterations and chromosomal rearrangements that can perturb protein function. Prime editing has been applied to install and evaluate genetic variants, but previous approaches have been limited by the variable efficiency of prime editing guide RNAs. Here we present a high-throughput prime editing sensor strategy that couples prime editing guide...

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Identifiers

PMID: 38472508
DOI: 10.1038/s41587-024-02172-9
PMCID: PMC12520993

Genes

TP53

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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