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Article

Massively parallel phenotyping of coding variants in cancer with Perturb-seq.

Nature biotechnology - 1 Jun 2022

Ursu Oana, Neal James T, Shea Emily, Thakore Pratiksha I, Jerby-Arnon Livnat, Nguyen Lan, Dionne Danielle, Diaz Celeste, Bauman Julia, Mosaad Mariam Mounir, Fagre Christian, Lo April, McSharry Maria, Giacomelli Andrew O, Ly Seav Huong, Rozenblatt-Rosen Orit, Hahn William C, Aguirre Andrew J, Berger Alice H, Regev Aviv, Boehm Jesse S

Abstract excerpt

Genome sequencing studies have identified millions of somatic variants in cancer, but it remains challenging to predict the phenotypic impact of most. Experimental approaches to distinguish impactful variants often use phenotypic assays that report on predefined gene-specific functional effects in bulk cell populations. Here, we develop an approach to functionally assess variant impact in single cells by pooled...

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PMID: 35058622
DOI: 10.1038/s41587-021-01160-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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