Article
A Systematic p53 Mutation Library Links Differential Functional Impact to Cancer Mutation Pattern and Evolutionary Conservation.
Molecular cell - 5 Jul 2018
Kotler Eran, Shani Odem, Goldfeld Guy, Lotan-Pompan Maya, Tarcic Ohad, Gershoni Anat, Hopf Thomas A, Marks Debora S, Oren Moshe, Segal Eran
Abstract excerpt
The TP53 gene is frequently mutated in human cancer. Research has focused predominantly on six major "hotspot" codons, which account for only ∼30% of cancer-associated p53 mutations. To comprehensively characterize the consequences of the p53 mutation spectrum, we created a synthetically designed library and measured the functional impact of ∼10,000 DNA-binding domain (DBD) p53 variants in human cells in culture...
Topics
- Animals
- Evolution, Molecular
- Gene Library
- HEK293 Cells
- Humans
- Mice
- Mice, Nude
- Mutation
- Neoplasms
- Polymorphism, Single Nucleotide
- Protein Domains