Article
Improved detection of colibactin-induced mutations by genotoxic E. coli in organoids and colorectal cancer.
Cancer cell - 11 Mar 2024
Rosendahl Huber Axel, Pleguezuelos-Manzano Cayetano, Puschhof Jens, Ubels Joske, Boot Charelle, Saftien Aurelia, Verheul Mark, Trabut Laurianne T, Groenen Niels, van Roosmalen Markus, Ouyang Kyanna S, Wood Henry, Quirke Phil, Meijer Gerrit, Cuppen Edwin, Clevers Hans, van Boxtel Ruben
Abstract excerpt
Co-culture of intestinal organoids with a colibactin-producing pks+E. coli strain (EcC) revealed mutational signatures also found in colorectal cancer (CRC). E. coli Nissle 1917 (EcN) remains a commonly used probiotic, despite harboring the pks operon and inducing double strand DNA breaks. We determine the mutagenicity of EcN and three CRC-derived pks+E. coli strains with an analytical framework based on sequence...
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