Article

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in <i>Candida</i> pathogens

2026-03-28

Abstract excerpt

<h4>ABSTRACT</h4> The auxin-inducible degron (AID) technology is a convenient and powerful tool for protein functional characterization in a broad array of eukaryotic species. We recently demonstrated that the original AID and improved AID2 systems are very effective at rapid protein depletion in Candida albicans and described a limited set of reagents for their use in certain auxotrophic lab strains. With an ey...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8763276f-df02-51b4-b700-504b1527f067
DOI: 10.64898/2026.03.27.714890

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in <i>Candida</i> pathogensDOI 10.64898/2026.03.27.714890Read

An auxin-inducible degron system for conditional mutation in the fungal meningitis pathogen Cryptococcus neoformans.Semantic proximity Read

Rapid, efficient auxin-inducible protein degradation in <i>Candida</i> pathogensSemantic proximity Read

Expanding the <i>Caenorhabditis elegans</i> auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editingSemantic proximity Read

Comparative analysis and directed protein evolution yield an improved degron technology with minimal basal degradation, rapid inducible depletion, and faster recovery of target proteins.Semantic proximity Read

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.Semantic proximity Read

HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cellsSemantic proximity Read

Engineering the auxin-inducible degron system for tunable <i>in vivo</i> control of organismal physiologySemantic proximity Read

HyperdCas12a-Based Multiplexed Genetic Regulation in <i>Candida albicans</i>Semantic proximity Read

Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinoleSemantic proximity Read

Redefining Pleiotropic Drug Resistance in a Pathogenic Yeast: Pdr1 Functions as a Sensor of Cellular Stresses in <i>Candida glabrata</i>Semantic proximity Read

One-step generation of auxin-inducible degron cells with high-efficiency homozygous taggingSemantic proximity Read

Identification of essential genes and fluconazole resistance genes in <i>Candida glabrata</i> by profiling of <i>Hermes</i> transposon insertionsSemantic proximity Read