Article
Highly Sensitive Enrichment of Low-Frequency Variants by Hairpin Competition Amplification.
Analytical chemistry - 15 Aug 2023
Liu Zhaocheng, Zhang Rui, Jiang Xixi, Ji Li, Sun Ping, Ji Yong, Zhang Yu, Ding Yan, Li Koukou, Pu Zhening, Zhou Fengsheng, Zou Jian
Abstract excerpt
Gene mutations are inevitably accumulated in cells of the human body. It is of great significance to detect mutations at the earliest possible time in physiological and pathological processes. However, genotyping low-copy tumor DNA (ctDNA) in patients is challenging due to abundant wild DNA backgrounds. One novel strategy to enrich rare mutations at low variant allele fractions (VAFs) with quantitative polymerase...
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