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Article

Detection of Ultralow-Frequency ctDNA Mutations Using a Dual Hairpin-Competition CRISPR/Cas14a System.

Analytical chemistry - 9 Jun 2026

Liu Zhaocheng, Zhang Rui, Chang Chunyan, Xu Daxing, Mao Dongsheng, Zhu Xiaoli, Xu Hongyang

Abstract excerpt

Circulating tumor DNA (ctDNA) mutation profiling is essential for guiding targeted therapy and monitoring cancer recurrence, yet its clinical adoption is constrained by overwhelming wild-type DNA background and the limited sensitivity of existing platforms. Here, we introduce a dual hairpin-competition CRISPR/Cas14a (DHCC) system that integrates two sequential layers of hairpin competition: selective enrichment...

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Identifiers

PMID: 42202254
DOI: 10.1021/acs.analchem.6c02495
PMCID: PMC13261614

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EGFR
NRAS
PAM

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