Article
A DddA ortholog-based and transactivator-assisted nuclear and mitochondrial cytosine base editors with expanded target compatibility.
Molecular cell - 18 May 2023
Guo Junfan, Yu Wenxia, Li Min, Chen Hongyu, Liu Jie, Xue Xiaowen, Lin Jianxiang, Huang Shisheng, Shu Wenjie, Huang Xingxu, Liu Zhen, Wang Shengqi, Qiao Yunbo
Abstract excerpt
Bacterial double-stranded DNA (dsDNA) cytosine deaminase DddAtox-derived cytosine base editor (DdCBE) and its evolved variant, DddA11, guided by transcription-activator-like effector (TALE) proteins, enable mitochondrial DNA (mtDNA) editing at TC or HC (H = A, C, or T) sequence contexts, while it remains relatively unattainable for GC targets. Here, we identified a dsDNA deaminase originated from a Roseburia...
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