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Article

High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase

8 Aug 2025

Abstract excerpt

CRISPR-based cytosine base editors enable precise genome editing without inducing double-stranded DNA breaks yet traditionally depend on a limited selection of deaminases from the APOBEC/AID or TadA families. Here, we present SsCBE, a CRISPR-based cytosine base editor utilizing SsdA tox , a DYW-like deaminase derived from the toxin of Pseudomonas syringae . Strategic engineering of SsdA tox has led to remarkable...

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PMID: 40781772
DOI: 10.1016/j.ymthe.2025.08.007

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