Article
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature - 1 Jul 2020
Mok Beverly Y, de Moraes Marcos H, Zeng Jun, Bosch Dustin E, Kotrys Anna V, Raguram Aditya, Hsu FoSheng, Radey Matthew C, Peterson S Brook, Mootha Vamsi K, Mougous Joseph D, Liu David R
Abstract excerpt
Bacterial toxins represent a vast reservoir of biochemical diversity that can be repurposed for biomedical applications. Such proteins include a group of predicted interbacterial toxins of the deaminase superfamily, members of which have found application in gene-editing techniques1,2. Because previously described cytidine deaminases operate on single-stranded nucleic acids3, their use in base editing requires...
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