Article
Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways.
Cell reports - 28 Feb 2023
Schimmel Joost, Muñoz-Subirana Núria, Kool Hanneke, van Schendel Robin, van der Vlies Sven, Kamp Juliette A, de Vrij Femke M S, Kushner Steven A, Smith Graeme C M, Boulton Simon J, Tijsterman Marcel
Abstract excerpt
Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by modulating DNA repair pathways holds considerable promise to increase the efficiency of genome engineering. Here, we show that inhibition of non-homologous end joining (NHEJ) or polymerase theta-mediated end joining (TMEJ) can be...
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