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Article

Development of a versatile high-throughput mutagenesis assay with multiplexed short-read NGS using DNA-barcoded supF shuttle vector library amplified in E. coli.

eLife - 10 Oct 2022

Kawai Hidehiko, Iwata Ren, Ebi Shungo, Sugihara Ryusei, Masuda Shogo, Fujiwara Chiho, Kimura Shingo, Kamiya Hiroyuki

Abstract excerpt

A forward mutagenesis assay using the supF gene has been widely employed for the last several decades in studies addressing mutation frequencies and mutation spectra associated with various intrinsic and environmental mutagens. In this study, by using a supF shuttle vector and non-SOS-induced Escherichia coli with short-read next-generation sequencing (NGS) technology, we present an advanced method for the study...

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Identifiers

PMID: 36214452
DOI: 10.7554/eLife.83780
PMCID: PMC9584611

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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