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Determination of single nucleotide variants in Escherichia coli DH5α by using short-read sequencing.

FEMS microbiology letters - 1 Jun 2015

Song Yoseb, Lee Bo-Rahm, Cho Suhyung, Cho Yoo-Bok, Kim Seon-Won, Kang Taek Jin, Kim Sun Chang, Cho Byung-Kwan

Abstract excerpt

Escherichia coli DH5α is a common laboratory strain that provides an important platform for routine use in cloning and synthetic biology applications. Many synthetic circuits have been constructed and successfully expressed in E. coli DH5α; however, its genome sequence has not been determined yet. Here, we determined E. coli DH5α genome sequence and identified genetic mutations that affect its phenotypic...

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PMID: 25934703
DOI: 10.1093/femsle/fnv073

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